Laboratory of Biopolymers
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Tbilisi State Medical University I.Kutateladze Institute of Pharmacochemistry

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Laboratory of Plant Biopolymers Departments


Area of research:

Subject matter of research of laboratory is isolation, structure elucidation of plant biopolymers (polysaccharides, novel phenolic polymer), synthesis of related compounds and investigation of their biological activity, namely study of immunomodulatory (anticomplementary), antioxidant, anti-inflammatory, wound-healing and anti-cancer properties. We isolated from the Caucasian species of comfrey Symphytum asperum and S.caucasicum (Boraginaceae) novel caffeic acid-derived polymer, namely poly[3-(3,4-dihydroxyphenyl)glyceric acid] or poly[oxy-1-carboxy-2-(3,4-dihydroxyphenyl)ethylene]. The polyoxyethylene chain is the backbone of this polymer molecule. Dihydroxyphenyl and carboxyl groups are regular substituents at two carbon atoms in the chain. Such biopolymer is hitherto not known and has been identified for the first time. This compound is a representative of a new class of natural polyethers with a residue of 3-(3,4-dihydroxyphenyl)glyceric acid as the repeating unit. 
This polymer possessed immunomodulatory (anticomplementary), antioxidant, antiinflammatory activities and modulated B-chronic lymphocytic leukaemia cell apoptosis and cell cicle progression. Besides, polymer completely abrogated the adhesion of murine B16 melanoma cells to tumor-activated hepatic sinusoidal endothelium (HSE) and prevented melanoma cell adherence to recombinant vascular endothelial growth factor (VEGF)-treated HSE.
The synthesis of 3-(3,4-dihydroxyphenyl)glyceric acid (DPGA), a monomer-racemate of poly[3-(3,4-dihydroxyphenyl)glyceric acid] as well as its two enantiomers was performed successfully. Such compound is not found in nature and was synthesized for the first time. 
Besides, we examined the anticancer efficacy of S. asperum and S.caucasicum phenolic polymers and DPGA in androgen dependent (LNCaP) and androgen independent (22Rv1 and PC3) prostate cancer (PCA) cells. These compounds (1-100 g/ml) significantly decreased the cell number and increased the cell death in all the three PCA cell lines after 24 and 48 h of treatment. But the relative efficacy of these three compounds on cell growth inhibition varied in this assay. S. asperum polymer was observed effective in all the three cell lines; while S.caucasicum polymer was most effective in LNCaP cells and DPGA in 22Rv1 cells. Fluorescence Activated Cell Sorting (FACS) analysis showed that the growth inhibition by these compounds was associated with a strong induction of cell cycle arrest in PCA cells. Overall, present study identified the strong efficacy of these novel phytochemicals against PCA cells, and suggested that their pre-clinical efficacy studies in PCA models are needed in future to move them forward for possible translational potential in PCA patients.

 

Laboratory has established tight research collaboration with the the Institute of Physical and Analytical Chemistry School of Exact and Natural Sciences at

Tbilisi State University (Director Prof. B.Chankvetadze, assoc. prof.  A. Mskhiladze)

 

Head of laboratory, Principal research scientist, D. Sci., Ph.D. Vakhtang Barbakadze
Senior research scientis: Ph.D. Lali Gogilashvili, Ph.D. Maia Merlani
Research scientist Ph.D. Lela Amiranashvili

Administration

 

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Laboratory of Steroids and Cardiac Glycosides

 

 

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Laboratory of Medicinal Fermentology

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Laboratory of Phenolic Compounds

 

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Laboratory of Terpene Compounds

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Laboratory of Plant Biopolymers

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Laboratory of Akaliods

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Laboratory of Lipids and Anthraquinones

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Laboratory of Pharmaceutical Technology

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Laboratory of Chemical Analysis and Standardisation

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Laboratory of Chemical Modification of Natural Compounds

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Laboratory of Pharmacobotany

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Department of Pharmacology

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Pilot Plant

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Department of the Resource Management

 

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Department of Scientific Information

 

 

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Administrative support center


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Storehouse

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